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Targeted intestinal deletion of Rho guanine nucleotide exchange factor 7, βPIX, impairs enterocyte proliferation, villus maturation, and mucosal defenses in mice

Am J Physiol Gastrointest Liver Physiol. 2021-02; 
Kunrong Cheng, Shannon M Larabee, Mazen Tolaymat, Marie Hanscom, Aaron C Shang, Alyssa Schledwitz, Shien Hu, Cinthia B Drachenberg, Min Zhan, Ahmed Chahdi, Jean-Pierre Raufman
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Custom DNA/RNA Oligos … cell migration which is important in normal physiology to maintain intestinal barrier function and 100 … under control of the Villin1 promoter [B6.SJL-Tg(Vil1-cre)997Gum/J, stock #004586, Jackson 141 Laboratory] (16) … (www.genscript.com/ssl-bin/app/primer) … Get A Quote

摘要

Rho guanine nucleotide exchange factors (RhoGEFs) regulate Rho GTPase activity and cytoskeletal and cell adhesion dynamics. βPix, a CDC42/RAC family RhoGEF encoded by is reported to modulate human colon cancer cell proliferation and postwounding restitution of rat intestinal epithelial monolayers. We hypothesized that βPix plays a role in maintaining intestinal epithelial homeostasis. To test this hypothesis, we examined βPix distribution in the human and murine intestine and created mice with intestinal epithelial-selective βPix deletion [βPix/Tg(villin-Cre); CKO mice]. Using conditional knockout (CKO) and control mice, we investigated the consequences of βPix deficiency in vivo on intestinal epitheli... More

关键词

Rho-associated protein kinase, cell proliferation, dextran sodium sulfate colitis, intestinal epithelial cells, intestinal organoids, β-catenin signaling