Products/Services Used | Details | Operation |
---|---|---|
Custom DNA/RNA Oligos> | … Toxin-specific primers were designed with the assistance of online primer design software (Genscript). The mucoricin gene primers used were: G7F1, 5′-CTGGCGTTACGAAAATGGTT-3′ and G7R1, 5′-TAAATCAGGACGGGCTTCAC-3′. The amplification efficiency was … | Get A Quote |
Fungi of the order Mucorales cause mucormycosis, a lethal infection with an incompletely understood pathogenesis. We demonstrate that Mucorales fungi produce a toxin, which plays a central role in virulence. Polyclonal antibodies against this toxin inhibit its ability to damage human cells in vitro and prevent hypovolemic shock, organ necrosis and death in mice with mucormycosis. Inhibition of the toxin in Rhizopus delemar through RNA interference compromises the ability of the fungus to damage host cells and attenuates virulence in mice. This 17 kDa toxin has structural and functional features of the plant toxin ricin, including the ability to inhibit protein synthesis through its N-glycosylase activity, the... More