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Bola3 Regulates Beige Adipocyte Thermogenesis Maintaining Mitochondrial Homeostasis and Lipolysis

Front Endocrinol (Lausanne). 2021-01; 
Ningning Bai, Jingyuan Ma, Miriayi Alimujiang, Jun Xu, Fan Hu, Yuejie Xu, Qingyang Leng, Shuqing Chen, Xiaohua Li, Junfeng Han, Weiping Jia, Yuqian Bao, Ying Yang
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Custom DNA/RNA Oligos … Quantitative expression of targeted genes was normalized to housekeeping gene 36B4 and calculated using the 2 −ΔΔCT method. The primer sequences are summarized in Table 1 … The antibody against BOLA3 was custom-produced by GenScript … Get A Quote

摘要

Mitochondrial iron-sulfur (Fe-S) cluster is an important cofactor for the maturation of Fe-S proteins, which are ubiquitously involved in energy metabolism; however, factors facilitating this process in beige fat have not been established. Here, we identified BolA family member 3 (Bola3), as one of 17 mitochondrial Fe-S cluster assembly genes, was the most significant induced gene in the browning program of white adipose tissue. Using lentiviral-delivered shRNA , we determined that Bola3 deficiency inhibited thermogenesis activity without affecting lipogenesis in differentiated beige adipocytes. The inhibition effect of Bola3 knockdown might be through impairing mitochondrial homeostasis and lipolysis. This was... More

关键词

BolA family member 3, beige adipocyte, lipolysis, mitochondria, thermogenesis