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Combined evolutionary engineering and genetic manipulation improve low pH tolerance and butanol production in a synthetic microbial Clostridium community

Biotechnology and bioengineering. 2020-07; 
Zhiqiang Wen , Rodrigo Ledesma-Amaro , Minrui Lu , Yu Jiang , Shuliang Gao , Mingjie Jin , Sheng Yang
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摘要

Synthetic microbial communities have become a focus of biotechnological research since they can overcome several of the limitations of single-specie cultures. A paradigmatic example is Clostridium cellulovorans DSM 743B, which can decompose lignocellulose but cannot produce butanol. Clostridium beijerinckii NCIMB 8052 however, is unable to use lignocellulose but can produce high amounts of butanol from simple sugars. In our previous studies, both organisms were cocultured to produce butanol by consolidated bioprocessing. However, such consolidated bioprocessing implementation strongly depends on pH regulation. Since low pH (pH 4.5-5.5) is required for butanol fermentation, C. cellulovorans cannot grow well and ... More

关键词

adaptive laboratory evolution; agmatine deiminase; cell wall lyases; consolidated bioprocessing; low pH resistance.