Products/Services Used | Details | Operation |
---|---|---|
PCR and Cloning> | … Universal qPCR Master Mix (NEB, Frankfurt, Germany). Primers were designed using “GenScript Real-Time PCR (TaqMan) Primer Design” tool (Gen Script, Piscataway, New Jersey, USA). Primer sequences are given in Table 3 … | Get A Quote |
, a member of the Ustilaginaceae family, is a promising host for the production of several metabolites including itaconic acid. This dicarboxylate has great potential as a bio-based building block in the polymer industry, and is of special interest for pharmaceutical applications. Several itaconate overproducing strains have been generated by metabolic and morphology engineering. This yielded stabilized unicellular morphology through deletion, reduction of by-product formation through deletion of genes responsible for itaconate oxidation and (glyco)lipid production, and the overexpression of the regulator of the itaconate cluster and the mitochondrial tricarboxylate transporter encoded by from . In this stu... More