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Influence of Diclofenac on Activated Sludge Bacterial Communities in Fed-Batch Reactors

Food Technol Biotechnol. 2020-12; 
Barbara Kraigher, Ines Mandic-Mulec
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DNA Sequencing … T-RFLP. PCR reactions were purified with QuickClean 5M PCR purification kit (GenScript USA Inc., Piscataway, NJ, USA) and sequenced by Macrogen Inc. (Seoul, Korea). Sequences of 700-800 bp were obtained. Some isolates … Get A Quote

摘要

unassigned: The occurrence and environmental toxicity of pharmaceuticals have recently attracted increasing attention. Diclofenac is a highly consumed non-steroidal anti-inflammatory drug, which is often detected in wastewaters, but investigations of its influence on bacteria are scarce. unassigned: We investigated the influence of this pharmaceutical on bacterial community in activated sludge exposed to increasing concentrations of diclofenac in fed-batch reactors over 41 days. Nitrification activity of the activated sludge was measured and changes in bacterial community structure were followed using culture-independent molecular method (terminal restriction fragment length polymorphism, T-RFLP) and by the cul... More

关键词

Pseudomonas, activated sludge, bacterial community T-RFLP, diclofenac, pharmaceuticals