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Monoclonal Antibody Services> | … Transferring gel stamp to an Immun-Blot ® PVDF membrane (Bio-Rad, Hercules, CA, United States) using eBlot ® protein transfer system (GenScript, Nanjing, China), samples underwent a standard Western-blot protocol with GFP (B-2) mouse monoclonal antibody (sc-9996 … | Get A Quote |
has been considered as the most used model bacteria in the majority of studies for several decades. However, a new, faster chassis for synthetic biology is emerging in the form of the fast-growing gram-negative bacterium . Different methodologies, well established in , are currently being adapted for in the hope to enable a much faster platform for general molecular biology studies. Amongst the vast technologies available for , genetic code expansion, the incorporation of unnatural amino acids into proteins, serves as a robust tool for protein engineering and biorthogonal modifications. Here we designed and adapted the genetic code expansion methodology for and demonstrate an unnatural amino acid incorporatio... More