Genetic Code Expansion of

has been considered as the most used model bacteria in the majority of studies for several decades. However, a new, faster chassis for synthetic biology is emerging in the form of the fast-growing gram-negative bacterium . Different methodologies, well established in , are currently being adapted for in the hope to enable a much faster platform for general molecular biology studies. Amongst the vast technologies available for , genetic code expansion, the incorporation of unnatural amino acids into proteins, serves as a robust tool for protein engineering and biorthogonal modifications. Here we designed and adapted the genetic code expansion methodology for and demonstrate an unnatural amino acid incorporation into a protein for the first time in this organism.