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Genetic Code Expansion of

Front Bioeng Biotechnol. 2021-02; 
Eden Ozer, Lital Alfonta
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Monoclonal Antibody Services … Transferring gel stamp to an Immun-Blot ® PVDF membrane (Bio-Rad, Hercules, CA, United States) using eBlot ® protein transfer system (GenScript, Nanjing, China), samples underwent a standard Western-blot protocol with GFP (B-2) mouse monoclonal antibody (sc-9996 … Get A Quote

摘要

has been considered as the most used model bacteria in the majority of studies for several decades. However, a new, faster chassis for synthetic biology is emerging in the form of the fast-growing gram-negative bacterium . Different methodologies, well established in , are currently being adapted for in the hope to enable a much faster platform for general molecular biology studies. Amongst the vast technologies available for , genetic code expansion, the incorporation of unnatural amino acids into proteins, serves as a robust tool for protein engineering and biorthogonal modifications. Here we designed and adapted the genetic code expansion methodology for and demonstrate an unnatural amino acid incorporatio... More

关键词

Vibrio natriegens, genetic code expansion, propargyl-L-lysine, synthetic biology, unnatural amino acids