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RNAi-mediated repression of dormancy-related genes results in evergrowing apple trees

Tree Physiol. 2021-02; 
Rongmei Wu, Janine Cooney, Sumathi Tomes, Ria Rebstock, Sakuntala Karunairetnam, Andrew C Allan, Richard C Macknight, Erika Varkonyi-Gasic
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PCR Cloning and Subcloning … transcripts. The fragment was synthesized by GenScript (GenScript, Inc., Piscataway, New Jersey, United States) and cloned in sense and antisense orientation in a GATEWAY cloning system-based RNAi binary vector, pTKO2 … Get A Quote

摘要

DORMANCY ASSOCIATED MADS-box (DAM) and SHORT VEGETATIVE PHASE (SVP) genes have been implicated in the regulation of winter dormancy in perennials. Ectopic expression of apple (Malus ×domestica 'Royal Gala') DAM and SVP genes delays budbreak and constrains lateral shoot outgrowth. In this study, we used RNAi interference (RNAi) to simultaneously target all apple DAM and SVP genes to study their role and mode of action in the regulation of bud dormancy, budbreak and flowering. A synthetic construct carrying a hairpin fragment assembled from sequences specific to coding regions of three DAM and two SVP genes was used to generate transgenic lines. Reduced expression of DAM/SVP genes resulted in delayed leaf senesc... More

关键词

SVP, DAM, RNAi, apple, budbreak, dormancy