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Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider

Data Brief. 2020-01; 
Hiroki Oda, Yasuko Akiyama-Oda
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Oligo pool … time-lapse microscopy (Movie S1). 2.2. Microarray analysis. 40-mer oligonucleotide probes designed were embedded in custom microarrays (CombiMatrix CustomArray 12K×2, CustomArray, Inc.). The same microarray design … Get A Quote

摘要

Simultaneous, parental RNA interference (pRNAi) mediated knockdown of Hedgehog and Decapentaplegic (Dpp) signaling components, () and , respectively, exhibited serious defects in the formation of the major embryonic axes in the model spider . This paper describes a dataset of a custom oligonucleotide two-color microarray analysis that was carried out to compare the transcript expression levels between untreated (normal) and  +  double pRNAi embryos at late stage 5. Array spots that showed the intensity ratio of [ +  double pRNAi]/[normal] <0.6 were categorized as positive. The expressions of most, not all, of the transcripts related to the positive array spots were examined in embryos by whole-mount hybri... More

关键词

Arthropod, Axis formation, Embryology, Emerging model organism, Microarray, Pattern formation, RNA interference, Signaling pathway