Products/Services Used | Details | Operation |
---|---|---|
Oligo pool> | … time-lapse microscopy (Movie S1). 2.2. Microarray analysis. 40-mer oligonucleotide probes designed were embedded in custom microarrays (CombiMatrix CustomArray 12K×2, CustomArray, Inc.). The same microarray design … | Get A Quote |
Simultaneous, parental RNA interference (pRNAi) mediated knockdown of Hedgehog and Decapentaplegic (Dpp) signaling components, () and , respectively, exhibited serious defects in the formation of the major embryonic axes in the model spider . This paper describes a dataset of a custom oligonucleotide two-color microarray analysis that was carried out to compare the transcript expression levels between untreated (normal) and + double pRNAi embryos at late stage 5. Array spots that showed the intensity ratio of [ + double pRNAi]/[normal] <0.6 were categorized as positive. The expressions of most, not all, of the transcripts related to the positive array spots were examined in embryos by whole-mount hybri... More