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Mutagenesis Services> | … Then, qPCR was performed using FastStart Universal SYBR Premix ExTaqTM II (Takara Biotechnology) on an ABI PRISM® 7900HT System (Applied … taining the putative wild- type (WT) or mutant (MUT) binding sites for AP- 1 were synthesized by Genscript Biotech Corporation … | Get A Quote |
Osteoporosis is one of the most common metabolic bone diseases affecting millions of people. We previously found that harmine prevents bone loss in ovariectomized mice via increasing preosteoclast platelet-derived growth factor-BB (PDGF-BB) production and type H vessel formation. However, the molecular mechanisms by which harmine promotes preosteoclast PDGF-BB generation are still unclear. In this study, we revealed that inhibitor of DNA binding-2 (Id2) and activator protein-1 (AP-1) were important factors implicated in harmine-enhanced preosteoclast PDGF-BB production. Exposure of RANKL-induced Primary bone marrow macrophages (BMMs), isolated from tibiae and femora of mice, to harmine increased the protein lev... More