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Harmine targets inhibitor of DNA binding-2 and activator protein-1 to promote preosteoclast PDGF-BB production

J Cell Mol Med. 2021-05; 
Jie Huang, You-You Li, Kun Xia, Yi-Yi Wang, Chun-Yuan Chen, Meng-Lu Chen, Jia Cao, Zheng-Zhao Liu, Zhen-Xing Wang, Hao Yin, Xiong-Ke Hu, Zheng-Guang Wang, Yong Zhou, Hui Xie
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Mutagenesis Services … Then, qPCR was performed using FastStart Universal SYBR Premix ExTaqTM II (Takara Biotechnology) on an ABI PRISM® 7900HT System (Applied … taining the putative wild- type (WT) or mutant (MUT) binding sites for AP- 1 were synthesized by Genscript Biotech Corporation … Get A Quote

摘要

Osteoporosis is one of the most common metabolic bone diseases affecting millions of people. We previously found that harmine prevents bone loss in ovariectomized mice via increasing preosteoclast platelet-derived growth factor-BB (PDGF-BB) production and type H vessel formation. However, the molecular mechanisms by which harmine promotes preosteoclast PDGF-BB generation are still unclear. In this study, we revealed that inhibitor of DNA binding-2 (Id2) and activator protein-1 (AP-1) were important factors implicated in harmine-enhanced preosteoclast PDGF-BB production. Exposure of RANKL-induced Primary bone marrow macrophages (BMMs), isolated from tibiae and femora of mice, to harmine increased the protein lev... More

关键词

AP-1, Id2, PDGF-BB, harmine, preosteoclast