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ORF cDNA Clones/MolecularCloud> | … The sequences used for the design of linear peptides of the ORF8 protein of SARS-CoV-2 is under NCBI Reference Sequence: YP_009724396.1. Preliminary epitope screening was used with a library of peptides (GenScript) consisting of 15-mer peptides overlapping by 10 … | Get A Quote |
Given the on-going SARS-CoV-2 pandemic, identification of immunogenic targets against the viral protein will provide crucial advances towards the development of sensitive diagnostic tools and vaccination strategies. Our previous study has found that ORF8 protein of SARS-CoV-2 is highly immunogenic and shows high sensitivity in identifying COVID-19 disease. In this study, by employing overlapping linear peptides, we characterized the IgG immunodominant regions on SARS-CoV-2 ORF8 protein that are seropositive in the sera from SARS-CoV-2-infected patients. The major immunogenic epitopes are localized at (1) N-termini alpha helix, (2) the resides spanning beta 2 and 3 sheets, and (3) the loop between beta 4 and 5 s... More