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Mediates Early Host Immune Responses via Macrophage and Toll-Like Receptor Pathways

Infect Immun. 2021-05; 
Mariam Bakshi, Deborah Hebert, Connor Gulbronson, Gary Bauchan, Wenbin Tuo, Dante Zarlenga
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Nucleic Acid Purification & Analysis … The pure OoESP-L4 and OoESP-Ad were analyzed on 8 to 16% bis-Tris SDS-PAGE gels (GenScript, Piscataway, NJ) under reducing conditions. Alternatively, homogenized whole-worm extracts from L4 and adult stages were compared with the OoESP-L4 and OoESP-Ad … Get A Quote

摘要

is an abomasal parasite with significant economic impact on the cattle industry. Early host immune responses are poorly understood. Here, we examined time course expression of Toll-like receptors (TLRs) in peripheral blood mononuclear cells (PBMC) during infection where PBMC macrophages (Mϕ) generated both pro- and anti-inflammatory responses when incubated with excretory/secretory products (ESP) from fourth-stage larvae (OoESP-L4) or adult worms (OoESP-Ad). First, changes in cell morphology clearly showed that both OoESP-L4 and OoESP-Ad activated PBMC-Mϕ , resulting in suppressed CD40 and increased CD80 expression. Expression of mRNAs for TLR1, -4, -5, and -7 peaked 7 days postinfection (dpi) (early L4), d... More

关键词

Ostertagia ostertagi, Toll-like receptor, anti-inflammatory, cytokine, excretory/secretory, host immune response, macrophages, peripheral blood mononuclear cells, proinflammatory