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Long noncoding RNA IRL regulates NF-κB-mediated immune responses through suppression of miR-27c-3p-dependent IRAK4 down-regulation in teleost fish

J Biol Chem. 2021-01; 
Weiwei Zheng, Qing Chu, Tianjun Xu
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Monoclonal Antibody Services … The antibody against IRAK4 was diluted at 1:1000 (Genscript); anti-FLAG, anti-GFP, and anti-Tubulin monoclonal antibodies were diluted at 1: 2000 (Beyotime); and horseradish peroxidase–conjugated anti-rabbit IgG or anti-mouse IgG (Abbkine) were diluted at 1: 5000 … Get A Quote

摘要

Growing pieces of evidences show that the long non-coding RNAs (lncRNAs) as new regulators participate in the regulation of various physiological and pathological processes. The study of lncRNA in lower invertebrates is still unclear compared with that in mammals. Here, we identified a novel lncRNA, termed IRAK4-related lncRNA (IRL), as a key regulator for innate immunity in teleost fish. We find that miR-27c-3p inhibits IRAK4 expression and thus weakens the NF-κB-mediated signaling pathway. Furthermore, the Gram-negative bacterium Vibrio anguillarum and lipopolysaccharide significantly up-regulated host lncRNA IRL expression. Results indicate that IRL functions as a competing endogenous RNA for miR-27c-3p to ... More

关键词

IRAK4, Long noncoding RNA, ceRNA, innate immune, microRNA