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Cytosolic Delivery of Thiolated Neoantigen Nano-Vaccine Combined with Immune Checkpoint Blockade to Boost Anti-Cancer T Cell Immunity

Adv Sci (Weinh). 2021-01; 
Da Zhang, Ziguo Lin, Ming Wu, Zhixiong Cai, Youshi Zheng, Lei He, Zhenli Li, Jie Zhou, Liqin Sun, Geng Chen, Yongyi Zeng, Juan Li, Jingfeng Liu, Huanghao Yang, Xiaolong Liu
Products/Services Used Details Operation
Neoantigen Peptide Service … Shanghai, China). Neoantigens (HTDAHAQAFAALFDSMH) were synthesized and purified by HPLC in GenScript USA Inc. Antibodies used in this study were pur- chased from BioLegend, Inc. (San Diego, USA). LysoTracker … Get A Quote

摘要

Although tumor-specific neoantigen-based cancer vaccines hold tremendous potential, it still faces low cross-presentation associated with severe degradation via endocytosis pathway. Herein, a thiolated nano-vaccine allowing direct cytosolic delivery of neoantigen and Toll like receptor 9 agonist CpG-ODN is developed. This approach is capable of bypassing the endo-/lysosome degradation, increasing uptake and local concentration of neoantigen and CpG-ODN to activate antigen-presenting cells, significantly strengthening the anti-cancer T-cell immunity. In vivo immunization with thiolated nano-vaccine enhanced the lymph organ homing and promoted the antigen presentation on dendritic cells, effectively inhibited tum... More

关键词

T‐cells immunity, cytosolic delivery, immune checkpoint, neoantigen, thiolated nano‐vaccine