至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

A massively parallel 3' UTR reporter assay reveals relationships between nucleotide content, sequence conservation, and mRNA destabilization

Genome Res. 2019-05; 
Adam J Litterman, Robin Kageyama, Olivier Le Tonqueze, Wenxue Zhao, John D Gagnon, Hani Goodarzi, David J Erle, K Mark Ansel
Products/Services Used Details Operation
Oligo pool … retroviral microRNA sensor plasmid (Steiner et al. 2011). A DNA oligonucleotide library consisting of RBP-occupied sequences and control sequences was synthesized by CustomArray. Five sets of control sequences were included … Get A Quote

摘要

Compared to coding sequences, untranslated regions of the transcriptome are not well conserved, and functional annotation of these sequences is challenging. Global relationships between nucleotide composition of 3' UTR sequences and their sequence conservation have been appreciated since mammalian genomes were first sequenced, but the functional relevance of these patterns remain unknown. We systematically measured the effect on gene expression of the sequences of more than 25,000 RNA-binding protein (RBP) binding sites in primary mouse T cells using a massively parallel reporter assay. GC-rich sequences were destabilizing of reporter mRNAs and come from more rapidly evolving regions of the genome. These sequen... More

关键词