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Establishment of a novel probe-based RT-qPCR approach for detection and quantification of tight junctions reveals age-related changes in the gut barriers of broiler chickens

PLoS ONE. 2021-03; 
J Sophia von Buchholz, Ivana Bilic, Jörg R Aschenbach, Michael Hess, Taniya Mitra, Wageha A Awad
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PCR and Cloning … Primers and probes targeting different TJ proteins were developed by the GenScript Biotech Real-time PCR (TaqMan) Primer Design Tool with default settings using the sequences available in the NCBI database (Table 1). During primer and probe design, attention was paid to … Get A Quote

摘要

Tight junctions (TJs) play a dominant role in gut barrier formation, therefore, resolving the structures of TJs in any animal species is crucial but of major importance in fast growing broilers. They are regulated in molecular composition, ultrastructure and function by intracellular proteins and the cytoskeleton. TJ proteins are classified according to their function into barrier-forming, scaffolding and pore-forming types with deductible consequences for permeability. In spite of their importance for gut health and its integrity limited studies have investigated the TJs in chickens, including the comprehensive evaluation of TJs molecular composition and function in the chicken gut. In the actual study sequenc... More

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