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piRNA-823 Is Involved in Cancer Stem Cell Regulation Through Altering DNA Methylation in Association With Luminal Breast Cancer

Front Cell Dev Biol. 2021-03; 
Xin Ding, Ya Li, Jinhui Lü, Qian Zhao, Yuefan Guo, Ziye Lu, Wenjing Ma, Pengfei Liu, Richard G Pestell, Chunli Liang, Zuoren Yu
Products/Services Used Details Operation
Custom DNA/RNA Oligos … Oligos and Transfection. All oligos for piRNA mimics or antisense inhibitors were synthesized by GenScript (Nanjing, China). The sequence for the piR-823 mimic is: 5′ r(AGCGUUGGUGGUAUAG UGGUGAGCAUAGCUGC … Get A Quote

摘要

Cancer stem cells (CSCs) are believed to be the main source of cancer relapse and metastasis. PIWI-interacting small non-coding RNAs (piRNAs) have been recently recognized to be relevant to cancer biology. Whether and how piRNAs regulate human CSCs remain unknown. Herein, upregulation of piR-823 was identified in tested luminal breast cancer cells, especially in the luminal subtype of breast CSCs. Enforced expression or targeted knockdown of piR-823 demonstrated its oncogenic function in regulating cell proliferation and colony formation in MCF-7 and T-47D breast cancer cells. In addition, piR-823 induced ALDH (+) breast CSC subpopulation promoted the expression of stem cell markers including OCT4, SOX2, KLF4, ... More

关键词

DNA methylation, breast cancer, cancer stem cell, non-coding RNAs, piR-823