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Micro-RNA-451 Reduces Proliferation of B-CPAP Human Papillary Thyroid Cancer Cells by Downregulating Expression of Activating Transcription Factor 2

Med Sci Monit. 2021-03; 
Mei-Feng Zhang, Zhe-Wei Fei, Lei Huang
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Mutagenesis Services … Luciferase Reporter Assay. The wild-type (WT) and mutant (MUT) 3′UTR of ATF2 were synthesized with Genscript (Nanjing, China) and inserted downstream of the luciferase reporter vector pGL3 (Promega, Madison, Wisconsin, United States) … Get A Quote

摘要

BACKGROUND MicroRNAs (miRNAs) are novel biomarkers that are important in tumorigenesis and cancer treatment resistance. miR-451 is expressed in human papillary thyroid carcinoma (PTC) tissues and is associated with tumor progression. This study investigated the molecular mechanism associated with the effects of miR-451 on B-CPAP human PTC cells in vitro. MATERIAL AND METHODS Binding of miRNAs to the 3' untranslated region (3'UTR) of messenger RNA (mRNA) was determined with a luciferase reporter assay. miRNAs and plasmids were transfected into human PTC B-CPAP cells with Lipofectamine 2000 Transfection Reagent. Cell viability was tested with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assa... More

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