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Evaluating a new mixed-mode resin Diamond MMC Mustang using Capto MMC ImpRes as a benchmark

Protein Expr Purif. 2021-06; 
Shengwei Zhang, Yan Wan, Jiwei Duan, Yifeng Li
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Nucleic Acid Purification & Analysis … Louis, MO, USA). Precast SurePAGE 4-12% gradient Bis-Tris gels, liquid container and eStain LG protein destainer were purchased from Genscript (Nanjing, China) … Non-reduced SDS-PAGE was performed using precast SurePAGE 4-12% gradient Bis-Tris gels from Genscript … Get A Quote

摘要

Diamond MMC Mustang is a relatively new mixed-mode resin, which mediates both cation exchange and hydrophobic interactions. In this work, we evaluated this resin using Cytiva's Capto MMC ImpRes, a well-established mixed-mode resin with similar properties, as a benchmark. The data suggest that in comparison with Capto MMC ImpRes, Diamond MMC Mustang exhibits comparable binding capacity and resolution. In addition, the resin under evaluation shows good lot-to-lot consistency. The information provided in this study allows users to have additional options when selecting mixed-mode resin for intermediate purification or final polishing, which is favourable especially at the present time when the supply chains of man... More

关键词

Bispecific antibody (bsAb), Capto MMC ImpRes, Diamond MMC Mustang, Mixed-mode resin