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Chromosome territory formation attenuates the translocation potential of cells

Elife. 2019-11; 
Leah F Rosin, Olivia Crocker, Randi L Isenhart, Son C Nguyen, Zhuxuan Xu, Eric F Joyce
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Oligo pool … Generation of whole-chromosome oligopaints Oligopaint libraries were designed as previously described, using the Oligoarray 2.1 software (Beliveau et al., 2012; Beliveau et al., 2018; Rosin et al., 2018) and the Dm3 genome build, and purchased from CustomArray … Get A Quote

摘要

The formation and spatial arrangement of chromosome territories (CTs) in interphase has been posited to influence the outcome and frequency of genomic translocations. This is supported by correlations between the frequency of inter-chromosomal contacts and translocation events in myriad systems. However, it remains unclear if CT formation itself influences the translocation potential of cells. We address this question in cells by modulating the level of Condensin II, which regulates CT organization. Using whole-chromosome Oligopaints to identify genomic rearrangements, we find that increased contact frequencies between chromosomes due to Condensin II knockdown leads to an increased propensity to form transloca... More

关键词

Condensin II, D. melanogaster, FISH, cell biology, chromosome territory, chromosomes, gene expression, genome integrity, oligopaints, translocation