Products/Services Used | Details | Operation |
---|---|---|
Oligo pool> | … Briefly, DNA templates for designs were synthesized (CustomArray, Bothell, WA) and sequenced on Illumina MiSeq instruments, and RNA was transcribed directly on the sequencing chip in a repurposed Illumina Genome Analyzer II instrument … | Get A Quote |
Riboswitches that couple binding of ligands to conformational changes offer sensors and control elements for RNA synthetic biology and medical biotechnology. However, design of these riboswitches has required expert intuition or software specialized to transcription or translation outputs; design has been particularly challenging for applications in which the riboswitch output cannot be amplified by other molecular machinery. We present a fully automated design method called RiboLogic for such "stand-alone" riboswitches and test it high-throughput experiments on 2875 molecules using RNA-MaP (RNA on a massively parallel array) technology. These molecules consistently modulate their affinity to the MS2 bacteriop... More