至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Automated Design of Diverse Stand-Alone Riboswitches

ACS Synth Biol. 2019-07; 
Michelle J Wu, Johan O L Andreasson, Wipapat Kladwang, William Greenleaf, Rhiju Das
Products/Services Used Details Operation
Oligo pool … Briefly, DNA templates for designs were synthesized (CustomArray, Bothell, WA) and sequenced on Illumina MiSeq instruments, and RNA was transcribed directly on the sequencing chip in a repurposed Illumina Genome Analyzer II instrument … Get A Quote

摘要

Riboswitches that couple binding of ligands to conformational changes offer sensors and control elements for RNA synthetic biology and medical biotechnology. However, design of these riboswitches has required expert intuition or software specialized to transcription or translation outputs; design has been particularly challenging for applications in which the riboswitch output cannot be amplified by other molecular machinery. We present a fully automated design method called RiboLogic for such "stand-alone" riboswitches and test it high-throughput experiments on 2875 molecules using RNA-MaP (RNA on a massively parallel array) technology. These molecules consistently modulate their affinity to the MS2 bacteriop... More

关键词

RNA, computer-assisted design, high-throughput measurements, molecular design, riboswitch, thermodynamic model