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Design principles for ligand-sensing, conformation-switching ribozymes

PLoS Comput Biol. 2009-12; 
Xi Chen, Andrew D Ellington
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Oligo pool … Briefly, DNA templates for designs were synthesized (CustomArray, Bothell, WA) and se- quenced on Illumina MiSeq instruments, and RNA was transcribed directly on the sequencing chip in a repurposed Illumina Genome Analyzer II instrument … Get A Quote

摘要

Nucleic acid sensor elements are proving increasingly useful in biotechnology and biomedical applications. A number of ligand-sensing, conformational-switching ribozymes (also known as allosteric ribozymes or aptazymes) have been generated by some combination of directed evolution or rational design. Such sensor elements typically fuse a molecular recognition domain (aptamer) with a catalytic signal generator (ribozyme). Although the rational design of aptazymes has begun to be explored, the relationships between the thermodynamics of aptazyme conformational changes and aptazyme performance in vitro and in vivo have not been examined in a quantitative framework. We have therefore developed a quantitative and pr... More

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