| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … al. 2000). CircRNA plasmid design. The expression cassette shown in Figure 1A was custom synthesized in pUC57 by GenScript and then subcloned into pCMV6-XL6 using EcoRI/XbaI sites to produce circV1.EMCV. To generate … | Get A Quote |
Circular (circ) RNA expression vectors are used as a method of identifying and characterizing RNA sequences that harbor internal ribosome entry site (IRES) activity. During the course of developing a vector series tailored for IRES discovery, we found evidence for the occurrence of -spliced mRNAs arising when sequences with promoter activity were embedded between the upstream CTD and downstream NTD exons of the pre-mRNA. These -spliced products regenerate the same open reading frame expected from a circRNA and can lead to false-positive signals in screens relying on circRNA expression vectors for IRES discovery. Our results caution against interpretations of IRES activity solely based on results obtained from c... More
