至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

The trypsin-enhanced infection of porcine epidemic diarrhea virus is determined by the S2 subunit of the spike glycoprotein

J Virol. 2021-03; 
Yubei Tan, Limeng Sun, Gang Wang, Yuejun Shi, Wanyu Dong, Yanan Fu, Zhen Fu, Huanchun Chen, Guiqing Peng
Products/Services Used Details Operation
DNA Sequencing … After determination of all the fragments by bacterial PCR, the positive clones were fur- ther determined by restriction fragment length polymorphism with KpnI, and the correct clone was des- ignated pBAC-PEDV-YN200 after sequencing (GenScript, Nanjing, China) … Get A Quote

摘要

Porcine epidemic diarrhea virus (PEDV) is an enteric pathogen in the swine industry, causing high mortality in neonatal piglets. Efficient PEDV infection usually relies on the presence of trypsin, yet the mechanism of trypsin dependency is ambiguous. Here, we identified two PEDV strains, trypsin-enhanced YN200 and trypsin-independent DR13, in which the spike (S) protein of YN200 exhibits a stronger ability to induce syncytium formation and cleaved by trypsin than that of DR13. Using a full-length infectious YN200 cDNA clone, we confirmed that the S protein is a trypsin dependency determinant by comparison of rYN200 and rYN200-S To explore the trypsin-associated sites of the YN200 S protein, we then constructed ... More

关键词