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The Atg16l1 gene: characterization of wild type, knock-in, and knock-out phenotypes in rats

Physiol Genomics. 2021-04; 
Kari Chesney, Hongsheng Men, Miriam A Hankins, Elizabeth C Bryda
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Nucleic Acid Purification & Analysis … 246 247 Protein Analysis: Proteins were separated using ExpressPlus™ PAGE gels (10x8 cm, 12%, 12 well; 248 GenScript, Jiangsu Province, China) at 140 V for 75 minutes following the manufacturer's protocol. Gel 249 electrophoresis … Get A Quote

摘要

ATG16L1 is a ubiquitous autophagy gene responsible, in part, for formation of the double-membrane bound autophagosome that delivers unwanted cellular debris and intracellular pathogens to the lysosome for degradation. A single, nonsynonymous adenine to guanine polymorphism resulting in a threonine to alanine amino acid substitution (T300A) directly preceded by a caspase cleavage site (DxxD) causes an increased susceptibility to Crohn's disease (CD) in humans. The mechanism behind this increased susceptibility is still being elucidated, however, the amino acid change caused by this point mutation results in increased ATG16L1 protein sensitivity to caspase 3-mediated cleavage. In order to generate novel rat strai... More

关键词

CRISPR, Crohn's diease, Genetic Models, Inflammatory Bowel Disease, Phenotyping