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Microarray data on the comparison of transcript expression between normal and RNAi embryos in the common house spider

Data Brief. 2019-08; 
Hiroki Oda, Yasuko Akiyama-Oda
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Oligo pool … 1. 2.1. Custom microarray design. 40-mer oligonucleotide probes were designed based on the accumulated P. tepidariorum EST and cDNA sequences [2], [3] using OligoArray 2.1 [4] and embedded in a custom microarray (CombiMatrix CustomArray 12K, CustomArray, Inc.) … Get A Quote

摘要

We conducted a custom microarray experiment to detect the differences in the transcript expression levels between untreated (normal) and -RNAi embryos at late stage 6 in the common house spider . The array probes were designed based on accumulated EST and cDNA sequences. The microarray dataset has been deposited in the Gene Expression Omnibus (GEO) Database at the National Center for Biotechnology Information (NCBI) under the accession GSE113064. The expression of the transcripts selected based on the detected differences was examined in embryos by whole-mount hybridization.

关键词

Arthropod, Embryogenesis, Gene expression, Notch signaling, Parasteatoda tepidariorum, Pattern formation