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A method for high-throughput production of sequence-verified DNA libraries and strain collections

Molecular systems biology. 2017-02; 
Smith JD, Schlecht U, Xu W, Suresh S, Horecka J, Proctor MJ, Aiyar RS, Bennett RA, Chu A, Li YF, Roy K, Davis RW, Steinmetz LM, Hyman RW, Levy SF, St Onge RP
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Oligo pool ). We obtained the probe library through commercial, array-based synthesis (CustomArray; Maurer et al were purchased as two separate oligonu- cleotide pools from CustomArray (both using the 12,472 CustomArray library, and computed the Coefficient of Variance (CoV, computed as the standard deviation in the amplified CustomArray library, and counted how many sequence-perfect probes were recovered in was generally amplified from 0.5 ll or 1 ll of array-synthesized library (CustomArray) using Get A Quote

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