Products/Services Used | Details | Operation |
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DNA Sequencing> | RNA probes labeled with 3'Cy3 with the following sequences were purchased from GenScript: GU Probe, 5'-UUGUUGUU-3'; Random Probe, 5'-GUUUAUGU-3',Initial optimization experiments were performed in 96- well black plates (Corning) using the TECAN spark plate reader. | Get A Quote |
CUGBP Elav-like family member 1 (CELF1), an RNA-binding protein (RBP), plays important roles in the pathogenesis of diseases such as myotonic dystrophy, liver fibrosis and cancers. However, targeting CELF1 is still a challenge, as RBPs are considered largely undruggable. Here, we discovered that compound 27 disrupted CELF1-RNA binding via structure-based virtual screening and biochemical assays. Compound 27 binds directly to CELF1 and competes with RNA for binding to CELF1. Compound 27 promotes IFN-γ secretion and suppresses TGF-β1-induced hepatic stellate cell (HSC) activation by inhibiting CELF1-mediated IFN-γ mRNA decay. In vivo, compound 27 attenuates CCl4-induced murine liver fibrosis. Furthermore, the ... More