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Nano-immobilization of PETase enzyme for enhanced polyethylene terephthalate biodegradation

Biochemical Engineering Journal. 2021-12; 
Yunpu Jia , Nadia A. Samak , Xuemi Hao , Zheng Chen , Gama Yang , Xuhao Zhao , Tingzhen Mu , Maohua Yang , Jianmin Xing
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DNA Sequencing The sequence of the resulting plasmids was confirmed using Sanger sequencing at GenScript China, Inc. Get A Quote

摘要

PET hydrolase (PETase), discovered in Ideonella sakaiensis, is a promising agent for the biodegradation of polyethylene terephthalate (PET) capable of PET decomposition under mild reaction conditions with limited stability and productivity. Here, the immobilization of His-tagged PETase was achieved by synthesizing enzymeinorganic nanoflowers, PETase@Co3(PO4)2, which was designed based on the principle of biomimetic mineralization. Immobilization of PETase onto nanostructured Co3(PO4)2 enjoys high enzyme loading and low mass transfer inhibition due to large specific surface area, high movement speed, and large surface curvature caused by small particle size. The nano-effect of inorganic carriers materialize ... More

关键词

Plastic biodegradation Immobilization of biocatalyst Biomimetic mineralization