Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | Recombinant human PPIB (amino acids 33–216), PPIC (amino acids 23–212), PPIEP (amino acids 138–301), PPIFP (amino acids 42–207), and PPIGP (amino acids 1–180) were synthesized and cloned into a pET-28a(+)-TEV vector by Genscript USA. | Get A Quote |
Tauopathies, such as Alzheimer's disease, are characterized by the misfolding and progressive accumulation of the microtubule associated protein tau. Chaperones, tasked with maintaining protein homeostasis, can become imbalanced with age and contribute to the progression of neurodegenerative disease. Cyclophilins are a promising pool of underinvestigated chaperones with peptidyl-prolyl isomerase activity that may play protective roles in regulating tau aggregation. Using a Thioflavin T fluorescence-based assay to monitor in vitro tau aggregation, all eight cyclophilins, which include PPIA to PPIH prevent tau aggregation, with PPIB, PPIC, PPID, and PPIH showing the greatest inhibition. The low thermal stability ... More