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Rice FLOURY ENDOSPERM22, encoding a pentatricopeptide repeat protein, is involved in both mitochondrial RNA splicing and editing and is crucial for endosperm development

J Integr Plant Biol. 2022-11; 
Hang Yang , Yunlong Wang , Yunlu Tian , Xuan Teng , Zehui Lv , Jie Lei , Erchao Duan , Hui Dong , Xue Yang , Yuanyan Zhang , Yinglun Sun , Xiaoli Chen , Xiuhao Bao , Rongbo Chen , Chuanwei Gu , Yipeng Zhang , Xiaokang Jiang , Wenyu Ma , Pengcheng Zhang , Yi Ji , Yu Zhang , Yihua Wang , Jianmin Wan
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摘要

Most of the reported P-type pentatricopeptide repeat (PPR) proteins play roles in organelle RNA stabilization and splicing. However, P-type PPRs involved in both RNA splicing and editing have rarely been reported, and their underlying mechanism remains largely unknown. Here, we report a rice floury endosperm22 (flo22) mutant with delayed amyloplast development in endosperm cells. Map-based cloning and complementation tests demonstrated that FLO22 encodes a mitochondrion-localized P-type PPR protein. Mutation of FLO22 resulting in defective trans-splicing of mitochondrial nad1 intron 1 and perhaps causing instability of mature transcripts affected assembly and activity of complex Ⅰ, and mitochondrial morpholog... More

关键词

Oryza sativa; PPR proteins; RNA editing; RNA splicing; endosperm development.