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PCR Cloning and Subcloning> | … coli and synthesized by GenScript, USA. We cloned ArchLec between NheI and HindIII sites … Absorbance at a wavelength of 280 nm was monitored after injecting 100 µL of ArchLec at a … | Get A Quote |
The crystal structures of an L-type lectin domain from Methanocaldococcus jannaschii in apo and mannose-bound forms have been determined. A thorough investigation of L-type lectin domains from several organisms provides insight into the differences in these domains from different kingdoms of life. While the overall fold of the L-type lectin domain is conserved, differences in the lengths of the carbohydrate-binding loops and significant variations in the Mn -binding site compared to the Ca -binding site are observed. Further, the sequence and phylogenetic analyses suggest that the archaeal L-type lectin domain is evolutionarily closer to the plant legume lectins than to its bacterial or animal counterparts. Thi... More