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Plasmid DNA Preparation> | … The sequence of the EXT domain from SlPEX1 (NCBI accession AF159296) was synthesized and cloned by GenScript into pUC57 (pUC57-EXT). The plasmid pBS-SS-TOM-EXT-LONG … | Get A Quote |
Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast and oscillatory growth is mediated by continuous remodeling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins, including extensins (EXTs). Class-III peroxidases (PRXs) are secreted into the apoplastic space and are thought to trigger either cell wall loosening or polymerization of cell wall components, such as Tyr-mediated assembly of EXT networks (EXT-PRXs). The precise role of these EXT-PRXs is unknown. Using genetic, biochemical, and modeling approaches, we identified and characterized three root-hair-specific putative EXT-PRXs, PRX01, PR... More