Products/Services Used | Details | Operation |
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Catalog Peptides> | … The CaM2 peptide (CNGB1 residues 1122–1143) was purchased from GenScript. The CaM2 peptide was added in threefold excess to Ca 2+ -bound CaM and concentrated to 0.4 mM … | Get A Quote |
Retinal cyclic nucleotide-gated (CNG) channels consist of two protein subunits (CNGA1 and CNGB1). Calmodulin (CaM) binds to two separate sites within the cytosolic region of CNGB1: CaM binding to an N-terminal site (human CNGB1 residues 565-587, called CaM1) decreases the open probability of CNG channels at elevated Ca levels in dark-adapted photoreceptors, whereas CaM binding to a separate C-terminal site (CNGB1 residues 1120-1147, called CaM2) may increase channel open probability in light activated photoreceptors. We recently reported NMR chemical shift assignments of Ca-saturated CaM bound to the CaM1 site of CNGB1 (BMRB no. 51222). Here, we report complete NMR chemical shift assignments of Ca-saturated CaM... More