至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Genetic engineering of the precursor supply pathway for the overproduction of the nC-surfactin isoform with promising MEOR applications

Microb Cell Fact. 2021-05; 
Fangxiang Hu, Weijie Cai, Junzhang Lin, Weidong Wang, Shuang Li
Products/Services Used Details Operation
Custom DNA/RNA Oligos … Oligonucleotide primers were synthesized by GenScript (Nanjing, China). The … The mixtures were vortexed at maximum speed (QT-2; Qite Corp., Shanghai, China), and then incubated … Get A Quote

摘要

background: Surfactin, a representative biosurfactant of lipopeptide mainly produced by Bacillus subtilis, consists of a cyclic heptapeptide linked to a β-hydroxy fatty acid chain. The functional activity of surfactin is closely related to the length and isomerism of the fatty acid chain. results: In this study, the fatty acid precursor supply pathway in Bacillus subtilis 168 for surfactin production was strengthened through two steps. Firstly, pathways competing for the precursors were eliminated with inactivation of pps and pks. Secondly, the plant medium-chain acyl-carrier protein (ACP) thioesterase (BTE) from Umbellularia californica was overexpressed. As a result, the surfactin titer after 24 h of cultiv... More

关键词

Bacillus subtilis, Lipopeptide, Microbial Enhanced Oil Recovery (MEOR), Surfactin, Thioesterase