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Nup98 Is Subverted from Annulate Lamellae by Hepatitis C Virus Core Protein to Foster Viral Assembly

MBio. 2022-03; 
Bertrand Boson, Chloé Mialon, Konstanze Schichl, Solène Denolly, François-Loïc Cosset
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Catalog Peptides … Synthetic peptides were manufactured by GenScript (Netherlands) and have been previously described (25). PEN was used as a control, SV40 NLS binds to IPOA5 (importin α5), HIV-1 … Get A Quote

摘要

Nup98, an essential component of the nuclear pore that also participates in annulate lamella pore structures localized in the cytosol, is involved in hepatitis C virus (HCV) assembly. Here, we combined confocal microscopy and biochemical assays to study the interplay between Nup98, core (i.e., the HCV capsid protein), and viral genomes. Our results show that in HCV-infected cells, core protein is necessary and sufficient to induce relocalization of Nup98 from annulate lamellae to lipid droplet-apposed areas in which core/NS5A and HCV genomic RNA [(+)RNA] are clustered to promote viral assembly. Furthermore, we found that Nup98 interacts with HCV RNA and that upon Nup98 downregulation, the viral (+)RNA genome wa... More

关键词

Nup98, annulate lamellae, assembly, hepatitis C virus, viral RNA