Products/Services Used | Details | Operation |
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Mutagenesis Services> | … with restriction sites BsmI/BamHI for LC (GenScript). The gene fragment of ustekinumab and … were generated by site directed mutagenesis (GenScript). The vectors containing HC and … | Get A Quote |
Monoclonal IgG antibodies are the fastest growing class of biologics, but large differences exist in their plasma half-life in humans. Thus, to design IgG antibodies with favorable pharmacokinetics, it is crucial to identify the determinants of such differences. Here, we demonstrate that the variable region sequences of IgG antibodies greatly affect cellular uptake and subsequent recycling and rescue from intracellular degradation by endothelial cells. When the variable sequences are masked by the cognate antigen, it influences both their transport behavior and binding to the neonatal Fc receptor (FcRn), a key regulator of IgG plasma half-life. Furthermore, we show how charge patch differences in the variable d... More