Products/Services Used | Details | Operation |
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ORF cDNA Clones/MolecularCloud> | … coli and the DNA synthesized by GenScript. This sequence was PCR amplified using … burgdorferi clone and DNA was isolated using a NucleoSpin tissue kit (Clontech Laboratories) per … | Get A Quote |
The Lyme disease agent, Borrelia burgdorferi, harbors a significantly reduced genome and relies on the scavenging of critical nutrients from its tick and mammalian hosts for survival. Riboflavin salvage has been shown to be important for B. burgdorferi infection of mice, yet the contributions of riboflavin to B. burgdorferi metabolism and survival in the tick remain unknown. Using a targeted mass spectrometry approach, we confirmed the importance of bb0318, the putative ATPase component of an ABC-type riboflavin transporter, for riboflavin salvage and the production of FMN and FAD. This analysis further revealed that Δbb0318 B. burgdorferi displayed increased levels of glycerol 3-phosphate compared to the wild... More