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Quantum Dot-Based Screening Identifies F3 Peptide and Reveals Cell Surface Nucleolin as a Therapeutic Target for Rhabdomyosarcoma

Cancers (Basel). 2022-10; 
Dzhangar Dzhumashev, Andrea Timpanaro, Safa Ali, Andrea J De Micheli, Kamel Mamchaoui, Ilaria Cascone, Jochen Rössler, Michele Bernasconi
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Custom DNA/RNA Oligos … Aptamer AS1411 [17] was synthesized by GenScript as a biotinylated oligonucleotide (Biotin-5’AminoC6-TTGGTGGTGGTGGTTGTGGTGGTGGTGG-3’), and all G were replaced with C … Get A Quote

摘要

Active drug delivery by tumor-targeting peptides is a promising approach to improve existing therapies for rhabdomyosarcoma (RMS), by increasing the therapeutic effect and decreasing the systemic toxicity, e.g., by drug-loaded peptide-targeted nanoparticles. Here, we tested 20 different tumor-targeting peptides for their ability to bind to two RMS cell lines, Rh30 and RD, using quantum dots Streptavidin and biotin-peptides conjugates as a model for nanoparticles. Four peptides revealed a very strong binding to RMS cells: NCAM-1-targeting NTP peptide, nucleolin-targeting F3 peptide, and two Furin-targeting peptides, TmR and shTmR. F3 peptide showed the strongest binding to all RMS cell lines tested, low binding ... More

关键词

F3 peptide, Saporin toxin, active targeting, aptamer, nucleolin, quantum dots, rhabdomyosarcoma