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Secretion of collagenases by Saccharomyces cerevisiae for collagen degradation

Biotechnol Biofuels Bioprod. 2022-08; 
Han Xiao, Xiufang Liu, Yunzi Feng, Lin Zheng, Mouming Zhao, Mingtao Huang
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Codon Optimization … Corresponding coding DNA sequences for ColG and ColH were codon-optimized and synthesized by GenScript. Standard methods in molecular biology, including PCR, fusion PCR, … Get A Quote

摘要

background: The production and processing of animal-based products generates many collagen-rich by-products, which have received attention both for exploitation to increase their added value and to reduce their negative environmental impact. The collagen-rich by-products can be hydrolyzed by collagenases for further utilization. Therefore, collagenases are of benefit for efficient collagen materials processing. An alternative and safe way to produce secreted collagenases is needed. results: Two collagenases from Hathewaya histolytica, ColG and ColH, were successfully secreted by the yeast Saccharomyces cerevisiae. Compared with the native signal peptide of collagenase, the α-factor leader is more efficient in ... More

关键词

Collagen degradation, Heterologous expression, Recombinant collagenases, Saccharomyces cerevisiae, Synergistic effect