Products/Services Used | Details | Operation |
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Catalog Peptides> | … Na + conduction, but the large cation YO-PRO-1 (MW of 376 Da) significantly reduces Na + … with 0.12 mg mL −1 FLAG peptide (GenScript). The elution was collected, concentrated, and … | Get A Quote |
Single particle cryo-EM often yields multiple protein conformations within a single dataset, but experimentally deducing the temporal relationship of these conformers within a conformational trajectory is not trivial. Here, we use thermal titration methods and cryo-EM in an attempt to obtain temporal resolution of the conformational trajectory of the vanilloid receptor TRPV1 with resiniferatoxin (RTx) bound. Based on our cryo-EM ensemble analysis, RTx binding to TRPV1 appears to induce intracellular gate opening first, followed by selectivity filter dilation, then pore loop rearrangement to reach the final open state. This apparent conformational wave likely arises from the concerted, stepwise, additive structu... More