Products/Services Used | Details | Operation |
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GenParts™ DNA Fragments | … To perform the screening mutation analysis, we ordered synthetic DNA fragments from GenScript and inserted them in the pTLM plasmid for amplified sequences or conducted directed … | Get A Quote |
Protein-coding genes in trypanosomes occur in polycistronic transcription units (PTUs). How RNA polymerase II (Pol II) initiates transcription of PTUs has not been resolved; the current model favors chromatin modifications inducing transcription rather than sequence-specific promoters. Here, we uncover core promoters by functional characterization of Pol II peaks identified by chromatin immunoprecipitation sequencing (ChIP-seq). Two distinct promoters are located between divergent PTUs, each driving unidirectional transcription. Detailed analysis identifies a 75-bp promoter that is necessary and sufficient to drive full reporter expression and contains functional motifs. Analysis of further promoters suggests t... More