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Identification of sequence-specific promoters driving polycistronic transcription initiation by RNA polymerase II in trypanosomes

Cell Rep. 2022-01; 
Carlos Cordon-Obras, Claudia Gomez-Liñan, Sara Torres-Rusillo, Isabel Vidal-Cobo, Diana Lopez-Farfan, Alicia Barroso-Del Jesus, Domingo Rojas-Barros, Mark Carrington, Miguel Navarro
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摘要

Protein-coding genes in trypanosomes occur in polycistronic transcription units (PTUs). How RNA polymerase II (Pol II) initiates transcription of PTUs has not been resolved; the current model favors chromatin modifications inducing transcription rather than sequence-specific promoters. Here, we uncover core promoters by functional characterization of Pol II peaks identified by chromatin immunoprecipitation sequencing (ChIP-seq). Two distinct promoters are located between divergent PTUs, each driving unidirectional transcription. Detailed analysis identifies a 75-bp promoter that is necessary and sufficient to drive full reporter expression and contains functional motifs. Analysis of further promoters suggests t... More

关键词

African trypanosomes, ChIP-seq, RNA polymerase II, core promoter, gene expression, nucleotide motifs, polycistronic transcription, transcription initiation site, transcriptional activity