Products/Services Used | Details | Operation |
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GenParts™ DNA Fragments> | … DNA fragments encoding the proteins of interest were synthesized by GenScript Biotech Corporation (Nanjing, China) and amplified by PCR with Phanta® Max Super-Fidelity DNA … | Get A Quote |
Peroxisome proliferator-activated receptor (PPAR)-γ is a key transcription activator controlling adipogenesis and lipid metabolism. PPARγ binds PPAR response elements (PPREs) as the obligate heterodimer with retinoid X receptor (RXR) α, but exactly how PPARγ orchestrates the transcriptional response is unknown. This study demonstrates that PPARγ forms phase-separated droplets in vitro and solid-like nuclear condensates in cell, which is intriguingly mediated by its DNA binding domain characterized by the zinc finger motif. Furthermore, PPARγ forms nuclear condensates at PPREs sites through phase separation to compartmentalize its heterodimer partner RXRα to initiate PPARγ-specific transcriptional activa... More