Products/Services Used | Details | Operation |
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Recombinant Antibody Expression> | … Peptides used for generation of recombinant BCRs are indicated (NA, not applicable). The … To generate FPQQPEQII-reactive B cells, we obtained synthetic DNA (GenScript) encoding a … | Get A Quote |
Antibodies to deamidated gluten peptides are accurate diagnostic markers of celiac disease. However, binding of patient antibodies to all possible gluten epitopes has not previously been investigated. Here, we assess serum antibody specificity across the gluten proteome by use of high-density peptide arrays. We confirm the importance of deamidation for antibody binding, and we show that the response is remarkably focused on the known epitope QPEQPFP (where E results from deamidation of Q). In addition, we describe an epitope in native (non-deamidated) gluten, QQPEQII (where E is gene encoded), which is associated with both B cell and T cell reactivity. Antibodies to this native epitope are cross-reactive with ... More