Products/Services Used | Details | Operation |
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Custom DNA/RNA Oligos> | … a molecular syringe for protein injection under the guidance of … Pnf and Pdp1 effectors were previously proven to be injected … serum using specific synthesized oligopeptides (Genscript). … | Get A Quote |
Extracellular contractile injection systems (eCISs) are widespread bacterial nanomachines that resemble T4 phage tail. As a typical eCIS, virulence cassette (PVC) was proposed to inject toxins into eukaryotic cells by puncturing the cell membrane from outside. This makes it an ideal tool for protein delivery in biomedical research. However, how to manipulate this nanocomplex as a molecular syringe is still undetermined. Here, we identify that one group of N-terminal signal peptide (SP) sequences are crucial for the effector loading into the inner tube of PVC complex. By application of genetic operation, cryo-electron microscopy, in vitro translocation assays, and animal experiments, we show that, under the gui... More