Products/Services Used | Details | Operation |
---|---|---|
Catalog Peptides> | … cascades (>10 7 variants/day) allowed us to dissect the intrinsic molecular features of the MKK-… All synthetic peptides were ordered from GenScript with purities >95%. The fluorescent … | Get A Quote |
The combination of ultrahigh-throughput screening and sequencing informs on function and intragenic epistasis within combinatorial protein mutant libraries. Establishing a droplet-based, in vitro compartmentalised approach for robust expression and screening of protein kinase cascades (>10 variants/day) allowed us to dissect the intrinsic molecular features of the MKK-ERK signalling pathway, without interference from endogenous cellular components. In a six-residue combinatorial library of the MKK1 docking domain, we identified 29,563 sequence permutations that allow MKK1 to efficiently phosphorylate and activate its downstream target kinase ERK2. A flexibly placed hydrophobic sequence motif emerges which is de... More