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Plasmid DNA Preparation> | … The gene encoding SPΔCDelta or SPΔCDelta-PD was synthesized (Genscript) and cloned into the pCAGGS plasmid, and each mutation was confirmed by sequencing. SARS-CoV-2-N… | Get A Quote |
The Delta variant had spread globally in 2021 and caused more serious disease than the original virus and Omicron variant. In this study, we investigated several virological features of Delta spike protein (SP), including protein maturation, its impact on viral entry of pseudovirus and cell-cell fusion, and its induction of inflammatory cytokine production in human macrophages and dendritic cells. The results showed that SPΔC exhibited enhanced S1/S2 cleavage in cells and pseudotyped virus-like particles (PVLPs). Further, SPΔC elevated pseudovirus entry in human lung cell lines and significantly enhanced syncytia formation. Furthermore, we revealed that SPΔC-PVLPs had stronger effects on stimulating NF-κB a... More