至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Disentangling the recognition complexity of a protein hub using a nanopore

Nat Commun. 2022-02; 
Lauren Ashley Mayse, Ali Imran, Motahareh Ghahari Larimi, Michael S Cosgrove, Aaron James Wolfe, Liviu Movileanu
Products/Services Used Details Operation
Plasmid DNA Preparation … A plasmid with omll4tfhua as the gene of interest was purchased from GenScript (Piscataway, NJ). This gene included a DNA sequence encoding, from the N to C terminus, a 13-… Get A Quote

摘要

WD40 repeat proteins are frequently involved in processing cell signaling and scaffolding large multi-subunit machineries. Despite their significance in physiological and disease-like conditions, their reversible interactions with other proteins remain modestly examined. Here, we show the development and validation of a protein nanopore for the detection and quantification of WD40 repeat protein 5 (WDR5), a chromatin-associated hub involved in epigenetic regulation of histone methylation. Our nanopore sensor is equipped with a 14-residue Win motif of mixed lineage leukemia 4 methyltransferase (MLL4), a WDR5 ligand. Our approach reveals a broad dynamic range of MLL4-WDR5 interactions and three distant subpopulat... More

关键词