Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | … A plasmid with omll4tfhua as the gene of interest was purchased from GenScript (Piscataway, NJ). This gene included a DNA sequence encoding, from the N to C terminus, a 13-… | Get A Quote |
WD40 repeat proteins are frequently involved in processing cell signaling and scaffolding large multi-subunit machineries. Despite their significance in physiological and disease-like conditions, their reversible interactions with other proteins remain modestly examined. Here, we show the development and validation of a protein nanopore for the detection and quantification of WD40 repeat protein 5 (WDR5), a chromatin-associated hub involved in epigenetic regulation of histone methylation. Our nanopore sensor is equipped with a 14-residue Win motif of mixed lineage leukemia 4 methyltransferase (MLL4), a WDR5 ligand. Our approach reveals a broad dynamic range of MLL4-WDR5 interactions and three distant subpopulat... More