Products/Services Used | Details | Operation |
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Plasmid DNA Preparation | … by Genscript and inserted into pMalc4x (NEB), generating a Maltose Binding Protein (MBP) tag. The plasmid encoding ferredoxin was synthesized by Genscript and … into a custom-made … | Get A Quote |
Iron-sulfur clusters are thought to be ancient cofactors that could have played a role in early protometabolic systems. Thus far, redox active, prebiotically plausible iron-sulfur clusters have always contained cysteine ligands to the cluster. However, extant iron-sulfur proteins can be found to exploit other modes of binding, including ligation by histidine residues, as seen with [2Fe-2S] Rieske and MitoNEET proteins. Here, we investigated the ability of cysteine- and histidine-containing peptides to coordinate a mononuclear Fe center and a [2Fe-2S] cluster and compare their properties with purified iron-sulfur proteins. The iron-sulfur peptides were characterized by UV-vis, circular dichroism, and paramagneti... More