Products/Services Used | Details | Operation |
---|---|---|
Catalog Peptides> | … MHC-I peptides used for refolding were prepared by chemical synthesis (Biopeptek Inc. or GenScript): photoA02 (KILGFVFJV, where J is the photolabile 3-amino-3-(2-nitrophenyl)-… | Get A Quote |
Chaperones tapasin and transporter associated with antigen processing (TAP)-binding protein related (TAPBPR) associate with the major histocompatibility complex (MHC)-related protein 1 (MR1) to promote trafficking and cell surface expression. However, the binding mechanism and ligand dependency of MR1/chaperone interactions remain incompletely characterized. Here in vitro, biochemical and computational studies reveal that, unlike MHC-I, TAPBPR recognizes MR1 in a ligand-independent manner owing to the absence of major structural changes in the MR1 α helix between empty and ligand-loaded molecules. Structural characterization using paramagnetic nuclear magnetic resonance experiments combined with restrained mol... More